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Susana’s research tools & skills

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Personal and social skills

creativity · initiative · calmness · empathy · interrelation with others · teamwork · active listening · stress control · resilient · analytical capacity · innovation · security · dialogue · participative · independent · collaborative

Fields of knowledge

protein biophysics · protein folding · protein structure · protein denaturation · intrinsically disordered proteins · linkers · protein aggregation · kinetics · spectroscopy · molecular dynamics · cloning · apoptosis · cancer · angiogenesis · Enviromental Chemistry · mercury · Ornithology · Statistics · aquatic systems · pollution · plant biology · education · divulgation

Nuclear Magnetic Resonance

sample preparation · shigemi tubes · experiment calibration, acquisition, process and assignment of 1D, 2D, 3D, 4D spectra · Bruker 600MHz Spectometer with and without cryoprobe · Paramagnetic Relaxation Enhancement · CYANA · NMR Pipe · X-PLOR · CcpNmr · CARA

Molecular Biology & protein expression and purification

Polymerase Chain Reaction · DNA electrophoresis in agarose gels · construction of recombinant vectors · transformation of competent cells · plating of bacteria in selective media · selection of recombinant bacterial strains · purification of plasmids · heterologous expression of recombinant proteins in different bacterial strains of E. coli · bacterial growth in selective media enriched in different magnetically active isotopes (1H, 13C and 15N) · expression test of heterologous proteins · protein electrophoresis in polyacrylamide gels (SDS-PAGE) and native gels · Western Blot · dialysis · bacterial lysis with French press, sonication and thermal shock · protein renaturation · ion exchange liquid chromatography · molecular exclusion chromatography · affinity chromatography

Characterization techniques

Nuclear Magnetic Resonance · Circular Dichroism · UV-Visible Spectroscopy · Fluorescence Spectroscopy · Liquid-liquid Extraction · Adsorption Chromatography · Atomic Absorption Spectroscopy · Solid Phase MicroExtraction and Gas Chromatography, and detection by Atomic Fluorescence Spectroscopy (SPME-GC-py-AFS) · Atomic Absorption

Computational biochemistry software

GROMACS · Campari · ProFASi · Flexible Meccano · CONCOORD · Rosetta · HADDOCK · PALES · Theseus · Amber tools · CRYSOL

Statistical software

SPSS · Stata · STATISTICA · Excel

Molecular representation and Art work

VMD · UCSF Chimera · MOLMOL · GIMP

Operating systems

Mac OS · Xubuntu · Ubuntu

Languages

Spanish (native) · Catalan (native) · English (basic user)

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